multi-array® plate consumables Search Results


multi-array® plate consumables  (Meso Scale Diagnostics LLC)
90
Meso Scale Diagnostics LLC multi-array® plate consumables
Multi Array® Plate Consumables, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/multi-array® plate consumables/product/Meso Scale Diagnostics LLC
Average 90 stars, based on 1 article reviews
multi-array® plate consumables - by Bioz Stars, 2026-06
90/100 stars
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consumable plates  (Molecular Instruments)
86
Molecular Instruments consumable plates
Diagram of a fully automated HCR workflow. (A) Automated sample processing using the Opentrons Flex robot. (A1) The <t>consumable</t> probe plate as the starting vessel. A 96-well plate, where each well contains 5 μl of a pool of probes for four mRNA targets is used as the starting vessel for sample processing. For all liquid handling steps throughout the protocol, dispense speeds are set at 200 μl/s and aspiration speeds at 10 μl/s. All aspirations are set to use a tip to well bottom distance of 3 mm. (A2) The probe hybridization step. 10 μl of fixed embryos in hybridization buffer are transferred to each well to start the probe hybridization step. The inset illustrates transferability of sea urchin embryos using standard automation pipette tips. (A3) The probe wash step. Probes are washed from the sample using a formamide-based probe wash buffer. The insets illustrate the liquid handling speeds and tip to bottom distances allow for resuspension and mixing of embryos throughout washes. (A4) The amplification step. 10 μl of embryos are transferred to a plate of pre-plated amplifier hairpins in dextran sulfate. (A5) The amplifier wash step. Amplifier hairpins are washed using 5×SSCT, and 150 μl of samples in 5×SSCT are subsequently transferred to a glass bottom high-content imaging plate. Samples are centered using a plate shaker module set at 450 rpm for 30 min. (B) Automated confocal image acquisition using the ImageXpress HT.ai. The imaging plates containing centered samples are placed into the confocal microscope. Depending on data storage availability, the center 4 or 9 are imaged for each well. An example of an output of the demultiplexed and multiplexed versions of a single site of a positive control sample well is shown. (C) A schematic of developmental stages imaged in this study to be used as a developmental staging key. PMCs, primary mesenchyme cells.
Consumable Plates, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/consumable plates/product/Molecular Instruments
Average 86 stars, based on 1 article reviews
consumable plates - by Bioz Stars, 2026-06
86/100 stars
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multiwell plate consumables  (Meso Scale Diagnostics LLC)
90
Meso Scale Diagnostics LLC multiwell plate consumables
Diagram of a fully automated HCR workflow. (A) Automated sample processing using the Opentrons Flex robot. (A1) The <t>consumable</t> probe plate as the starting vessel. A 96-well plate, where each well contains 5 μl of a pool of probes for four mRNA targets is used as the starting vessel for sample processing. For all liquid handling steps throughout the protocol, dispense speeds are set at 200 μl/s and aspiration speeds at 10 μl/s. All aspirations are set to use a tip to well bottom distance of 3 mm. (A2) The probe hybridization step. 10 μl of fixed embryos in hybridization buffer are transferred to each well to start the probe hybridization step. The inset illustrates transferability of sea urchin embryos using standard automation pipette tips. (A3) The probe wash step. Probes are washed from the sample using a formamide-based probe wash buffer. The insets illustrate the liquid handling speeds and tip to bottom distances allow for resuspension and mixing of embryos throughout washes. (A4) The amplification step. 10 μl of embryos are transferred to a plate of pre-plated amplifier hairpins in dextran sulfate. (A5) The amplifier wash step. Amplifier hairpins are washed using 5×SSCT, and 150 μl of samples in 5×SSCT are subsequently transferred to a glass bottom high-content imaging plate. Samples are centered using a plate shaker module set at 450 rpm for 30 min. (B) Automated confocal image acquisition using the ImageXpress HT.ai. The imaging plates containing centered samples are placed into the confocal microscope. Depending on data storage availability, the center 4 or 9 are imaged for each well. An example of an output of the demultiplexed and multiplexed versions of a single site of a positive control sample well is shown. (C) A schematic of developmental stages imaged in this study to be used as a developmental staging key. PMCs, primary mesenchyme cells.
Multiwell Plate Consumables, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/multiwell plate consumables/product/Meso Scale Diagnostics LLC
Average 90 stars, based on 1 article reviews
multiwell plate consumables - by Bioz Stars, 2026-06
90/100 stars
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tl-6 plates  (CN Bio Innovations)
90
CN Bio Innovations tl-6 plates
Diagram of a fully automated HCR workflow. (A) Automated sample processing using the Opentrons Flex robot. (A1) The <t>consumable</t> probe plate as the starting vessel. A 96-well plate, where each well contains 5 μl of a pool of probes for four mRNA targets is used as the starting vessel for sample processing. For all liquid handling steps throughout the protocol, dispense speeds are set at 200 μl/s and aspiration speeds at 10 μl/s. All aspirations are set to use a tip to well bottom distance of 3 mm. (A2) The probe hybridization step. 10 μl of fixed embryos in hybridization buffer are transferred to each well to start the probe hybridization step. The inset illustrates transferability of sea urchin embryos using standard automation pipette tips. (A3) The probe wash step. Probes are washed from the sample using a formamide-based probe wash buffer. The insets illustrate the liquid handling speeds and tip to bottom distances allow for resuspension and mixing of embryos throughout washes. (A4) The amplification step. 10 μl of embryos are transferred to a plate of pre-plated amplifier hairpins in dextran sulfate. (A5) The amplifier wash step. Amplifier hairpins are washed using 5×SSCT, and 150 μl of samples in 5×SSCT are subsequently transferred to a glass bottom high-content imaging plate. Samples are centered using a plate shaker module set at 450 rpm for 30 min. (B) Automated confocal image acquisition using the ImageXpress HT.ai. The imaging plates containing centered samples are placed into the confocal microscope. Depending on data storage availability, the center 4 or 9 are imaged for each well. An example of an output of the demultiplexed and multiplexed versions of a single site of a positive control sample well is shown. (C) A schematic of developmental stages imaged in this study to be used as a developmental staging key. PMCs, primary mesenchyme cells.
Tl 6 Plates, supplied by CN Bio Innovations, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tl-6 plates/product/CN Bio Innovations
Average 90 stars, based on 1 article reviews
tl-6 plates - by Bioz Stars, 2026-06
90/100 stars
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lc-12 plates  (CN Bio Innovations)
90
CN Bio Innovations lc-12 plates
Diagram of a fully automated HCR workflow. (A) Automated sample processing using the Opentrons Flex robot. (A1) The <t>consumable</t> probe plate as the starting vessel. A 96-well plate, where each well contains 5 μl of a pool of probes for four mRNA targets is used as the starting vessel for sample processing. For all liquid handling steps throughout the protocol, dispense speeds are set at 200 μl/s and aspiration speeds at 10 μl/s. All aspirations are set to use a tip to well bottom distance of 3 mm. (A2) The probe hybridization step. 10 μl of fixed embryos in hybridization buffer are transferred to each well to start the probe hybridization step. The inset illustrates transferability of sea urchin embryos using standard automation pipette tips. (A3) The probe wash step. Probes are washed from the sample using a formamide-based probe wash buffer. The insets illustrate the liquid handling speeds and tip to bottom distances allow for resuspension and mixing of embryos throughout washes. (A4) The amplification step. 10 μl of embryos are transferred to a plate of pre-plated amplifier hairpins in dextran sulfate. (A5) The amplifier wash step. Amplifier hairpins are washed using 5×SSCT, and 150 μl of samples in 5×SSCT are subsequently transferred to a glass bottom high-content imaging plate. Samples are centered using a plate shaker module set at 450 rpm for 30 min. (B) Automated confocal image acquisition using the ImageXpress HT.ai. The imaging plates containing centered samples are placed into the confocal microscope. Depending on data storage availability, the center 4 or 9 are imaged for each well. An example of an output of the demultiplexed and multiplexed versions of a single site of a positive control sample well is shown. (C) A schematic of developmental stages imaged in this study to be used as a developmental staging key. PMCs, primary mesenchyme cells.
Lc 12 Plates, supplied by CN Bio Innovations, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lc-12 plates/product/CN Bio Innovations
Average 90 stars, based on 1 article reviews
lc-12 plates - by Bioz Stars, 2026-06
90/100 stars
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consumables  (Sarstedt)
86
Sarstedt consumables
Diagram of a fully automated HCR workflow. (A) Automated sample processing using the Opentrons Flex robot. (A1) The <t>consumable</t> probe plate as the starting vessel. A 96-well plate, where each well contains 5 μl of a pool of probes for four mRNA targets is used as the starting vessel for sample processing. For all liquid handling steps throughout the protocol, dispense speeds are set at 200 μl/s and aspiration speeds at 10 μl/s. All aspirations are set to use a tip to well bottom distance of 3 mm. (A2) The probe hybridization step. 10 μl of fixed embryos in hybridization buffer are transferred to each well to start the probe hybridization step. The inset illustrates transferability of sea urchin embryos using standard automation pipette tips. (A3) The probe wash step. Probes are washed from the sample using a formamide-based probe wash buffer. The insets illustrate the liquid handling speeds and tip to bottom distances allow for resuspension and mixing of embryos throughout washes. (A4) The amplification step. 10 μl of embryos are transferred to a plate of pre-plated amplifier hairpins in dextran sulfate. (A5) The amplifier wash step. Amplifier hairpins are washed using 5×SSCT, and 150 μl of samples in 5×SSCT are subsequently transferred to a glass bottom high-content imaging plate. Samples are centered using a plate shaker module set at 450 rpm for 30 min. (B) Automated confocal image acquisition using the ImageXpress HT.ai. The imaging plates containing centered samples are placed into the confocal microscope. Depending on data storage availability, the center 4 or 9 are imaged for each well. An example of an output of the demultiplexed and multiplexed versions of a single site of a positive control sample well is shown. (C) A schematic of developmental stages imaged in this study to be used as a developmental staging key. PMCs, primary mesenchyme cells.
Consumables, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/consumables/product/Sarstedt
Average 86 stars, based on 1 article reviews
consumables - by Bioz Stars, 2026-06
86/100 stars
  Buy from Supplier
384 well plate consumables  (Corning Life Sciences)
90
Corning Life Sciences 384 well plate consumables
Diagram of a fully automated HCR workflow. (A) Automated sample processing using the Opentrons Flex robot. (A1) The <t>consumable</t> probe plate as the starting vessel. A 96-well plate, where each well contains 5 μl of a pool of probes for four mRNA targets is used as the starting vessel for sample processing. For all liquid handling steps throughout the protocol, dispense speeds are set at 200 μl/s and aspiration speeds at 10 μl/s. All aspirations are set to use a tip to well bottom distance of 3 mm. (A2) The probe hybridization step. 10 μl of fixed embryos in hybridization buffer are transferred to each well to start the probe hybridization step. The inset illustrates transferability of sea urchin embryos using standard automation pipette tips. (A3) The probe wash step. Probes are washed from the sample using a formamide-based probe wash buffer. The insets illustrate the liquid handling speeds and tip to bottom distances allow for resuspension and mixing of embryos throughout washes. (A4) The amplification step. 10 μl of embryos are transferred to a plate of pre-plated amplifier hairpins in dextran sulfate. (A5) The amplifier wash step. Amplifier hairpins are washed using 5×SSCT, and 150 μl of samples in 5×SSCT are subsequently transferred to a glass bottom high-content imaging plate. Samples are centered using a plate shaker module set at 450 rpm for 30 min. (B) Automated confocal image acquisition using the ImageXpress HT.ai. The imaging plates containing centered samples are placed into the confocal microscope. Depending on data storage availability, the center 4 or 9 are imaged for each well. An example of an output of the demultiplexed and multiplexed versions of a single site of a positive control sample well is shown. (C) A schematic of developmental stages imaged in this study to be used as a developmental staging key. PMCs, primary mesenchyme cells.
384 Well Plate Consumables, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/384 well plate consumables/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
384 well plate consumables - by Bioz Stars, 2026-06
90/100 stars
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culture flasks  (Becton Dickinson)
90
Becton Dickinson culture flasks
Diagram of a fully automated HCR workflow. (A) Automated sample processing using the Opentrons Flex robot. (A1) The <t>consumable</t> probe plate as the starting vessel. A 96-well plate, where each well contains 5 μl of a pool of probes for four mRNA targets is used as the starting vessel for sample processing. For all liquid handling steps throughout the protocol, dispense speeds are set at 200 μl/s and aspiration speeds at 10 μl/s. All aspirations are set to use a tip to well bottom distance of 3 mm. (A2) The probe hybridization step. 10 μl of fixed embryos in hybridization buffer are transferred to each well to start the probe hybridization step. The inset illustrates transferability of sea urchin embryos using standard automation pipette tips. (A3) The probe wash step. Probes are washed from the sample using a formamide-based probe wash buffer. The insets illustrate the liquid handling speeds and tip to bottom distances allow for resuspension and mixing of embryos throughout washes. (A4) The amplification step. 10 μl of embryos are transferred to a plate of pre-plated amplifier hairpins in dextran sulfate. (A5) The amplifier wash step. Amplifier hairpins are washed using 5×SSCT, and 150 μl of samples in 5×SSCT are subsequently transferred to a glass bottom high-content imaging plate. Samples are centered using a plate shaker module set at 450 rpm for 30 min. (B) Automated confocal image acquisition using the ImageXpress HT.ai. The imaging plates containing centered samples are placed into the confocal microscope. Depending on data storage availability, the center 4 or 9 are imaged for each well. An example of an output of the demultiplexed and multiplexed versions of a single site of a positive control sample well is shown. (C) A schematic of developmental stages imaged in this study to be used as a developmental staging key. PMCs, primary mesenchyme cells.
Culture Flasks, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/culture flasks/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
culture flasks - by Bioz Stars, 2026-06
90/100 stars
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endotoxin-free consumables  (Corning Life Sciences)
90
Corning Life Sciences endotoxin-free consumables
Diagram of a fully automated HCR workflow. (A) Automated sample processing using the Opentrons Flex robot. (A1) The <t>consumable</t> probe plate as the starting vessel. A 96-well plate, where each well contains 5 μl of a pool of probes for four mRNA targets is used as the starting vessel for sample processing. For all liquid handling steps throughout the protocol, dispense speeds are set at 200 μl/s and aspiration speeds at 10 μl/s. All aspirations are set to use a tip to well bottom distance of 3 mm. (A2) The probe hybridization step. 10 μl of fixed embryos in hybridization buffer are transferred to each well to start the probe hybridization step. The inset illustrates transferability of sea urchin embryos using standard automation pipette tips. (A3) The probe wash step. Probes are washed from the sample using a formamide-based probe wash buffer. The insets illustrate the liquid handling speeds and tip to bottom distances allow for resuspension and mixing of embryos throughout washes. (A4) The amplification step. 10 μl of embryos are transferred to a plate of pre-plated amplifier hairpins in dextran sulfate. (A5) The amplifier wash step. Amplifier hairpins are washed using 5×SSCT, and 150 μl of samples in 5×SSCT are subsequently transferred to a glass bottom high-content imaging plate. Samples are centered using a plate shaker module set at 450 rpm for 30 min. (B) Automated confocal image acquisition using the ImageXpress HT.ai. The imaging plates containing centered samples are placed into the confocal microscope. Depending on data storage availability, the center 4 or 9 are imaged for each well. An example of an output of the demultiplexed and multiplexed versions of a single site of a positive control sample well is shown. (C) A schematic of developmental stages imaged in this study to be used as a developmental staging key. PMCs, primary mesenchyme cells.
Endotoxin Free Consumables, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/endotoxin-free consumables/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
endotoxin-free consumables - by Bioz Stars, 2026-06
90/100 stars
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disposable heat-resistant plate covers  (Reynolds Consumer Products Inc)
90
Reynolds Consumer Products Inc disposable heat-resistant plate covers
Diagram of a fully automated HCR workflow. (A) Automated sample processing using the Opentrons Flex robot. (A1) The <t>consumable</t> probe plate as the starting vessel. A 96-well plate, where each well contains 5 μl of a pool of probes for four mRNA targets is used as the starting vessel for sample processing. For all liquid handling steps throughout the protocol, dispense speeds are set at 200 μl/s and aspiration speeds at 10 μl/s. All aspirations are set to use a tip to well bottom distance of 3 mm. (A2) The probe hybridization step. 10 μl of fixed embryos in hybridization buffer are transferred to each well to start the probe hybridization step. The inset illustrates transferability of sea urchin embryos using standard automation pipette tips. (A3) The probe wash step. Probes are washed from the sample using a formamide-based probe wash buffer. The insets illustrate the liquid handling speeds and tip to bottom distances allow for resuspension and mixing of embryos throughout washes. (A4) The amplification step. 10 μl of embryos are transferred to a plate of pre-plated amplifier hairpins in dextran sulfate. (A5) The amplifier wash step. Amplifier hairpins are washed using 5×SSCT, and 150 μl of samples in 5×SSCT are subsequently transferred to a glass bottom high-content imaging plate. Samples are centered using a plate shaker module set at 450 rpm for 30 min. (B) Automated confocal image acquisition using the ImageXpress HT.ai. The imaging plates containing centered samples are placed into the confocal microscope. Depending on data storage availability, the center 4 or 9 are imaged for each well. An example of an output of the demultiplexed and multiplexed versions of a single site of a positive control sample well is shown. (C) A schematic of developmental stages imaged in this study to be used as a developmental staging key. PMCs, primary mesenchyme cells.
Disposable Heat Resistant Plate Covers, supplied by Reynolds Consumer Products Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/disposable heat-resistant plate covers/product/Reynolds Consumer Products Inc
Average 90 stars, based on 1 article reviews
disposable heat-resistant plate covers - by Bioz Stars, 2026-06
90/100 stars
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cellulose acylate films fujitac zrd40  (FUJIFILM)
90
FUJIFILM cellulose acylate films fujitac zrd40
Diagram of a fully automated HCR workflow. (A) Automated sample processing using the Opentrons Flex robot. (A1) The <t>consumable</t> probe plate as the starting vessel. A 96-well plate, where each well contains 5 μl of a pool of probes for four mRNA targets is used as the starting vessel for sample processing. For all liquid handling steps throughout the protocol, dispense speeds are set at 200 μl/s and aspiration speeds at 10 μl/s. All aspirations are set to use a tip to well bottom distance of 3 mm. (A2) The probe hybridization step. 10 μl of fixed embryos in hybridization buffer are transferred to each well to start the probe hybridization step. The inset illustrates transferability of sea urchin embryos using standard automation pipette tips. (A3) The probe wash step. Probes are washed from the sample using a formamide-based probe wash buffer. The insets illustrate the liquid handling speeds and tip to bottom distances allow for resuspension and mixing of embryos throughout washes. (A4) The amplification step. 10 μl of embryos are transferred to a plate of pre-plated amplifier hairpins in dextran sulfate. (A5) The amplifier wash step. Amplifier hairpins are washed using 5×SSCT, and 150 μl of samples in 5×SSCT are subsequently transferred to a glass bottom high-content imaging plate. Samples are centered using a plate shaker module set at 450 rpm for 30 min. (B) Automated confocal image acquisition using the ImageXpress HT.ai. The imaging plates containing centered samples are placed into the confocal microscope. Depending on data storage availability, the center 4 or 9 are imaged for each well. An example of an output of the demultiplexed and multiplexed versions of a single site of a positive control sample well is shown. (C) A schematic of developmental stages imaged in this study to be used as a developmental staging key. PMCs, primary mesenchyme cells.
Cellulose Acylate Films Fujitac Zrd40, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cellulose acylate films fujitac zrd40/product/FUJIFILM
Average 90 stars, based on 1 article reviews
cellulose acylate films fujitac zrd40 - by Bioz Stars, 2026-06
90/100 stars
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six-well plates  (Advangene Consumables)
90
Advangene Consumables six-well plates
Diagram of a fully automated HCR workflow. (A) Automated sample processing using the Opentrons Flex robot. (A1) The <t>consumable</t> probe plate as the starting vessel. A 96-well plate, where each well contains 5 μl of a pool of probes for four mRNA targets is used as the starting vessel for sample processing. For all liquid handling steps throughout the protocol, dispense speeds are set at 200 μl/s and aspiration speeds at 10 μl/s. All aspirations are set to use a tip to well bottom distance of 3 mm. (A2) The probe hybridization step. 10 μl of fixed embryos in hybridization buffer are transferred to each well to start the probe hybridization step. The inset illustrates transferability of sea urchin embryos using standard automation pipette tips. (A3) The probe wash step. Probes are washed from the sample using a formamide-based probe wash buffer. The insets illustrate the liquid handling speeds and tip to bottom distances allow for resuspension and mixing of embryos throughout washes. (A4) The amplification step. 10 μl of embryos are transferred to a plate of pre-plated amplifier hairpins in dextran sulfate. (A5) The amplifier wash step. Amplifier hairpins are washed using 5×SSCT, and 150 μl of samples in 5×SSCT are subsequently transferred to a glass bottom high-content imaging plate. Samples are centered using a plate shaker module set at 450 rpm for 30 min. (B) Automated confocal image acquisition using the ImageXpress HT.ai. The imaging plates containing centered samples are placed into the confocal microscope. Depending on data storage availability, the center 4 or 9 are imaged for each well. An example of an output of the demultiplexed and multiplexed versions of a single site of a positive control sample well is shown. (C) A schematic of developmental stages imaged in this study to be used as a developmental staging key. PMCs, primary mesenchyme cells.
Six Well Plates, supplied by Advangene Consumables, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/six-well plates/product/Advangene Consumables
Average 90 stars, based on 1 article reviews
six-well plates - by Bioz Stars, 2026-06
90/100 stars
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Image Search Results


Diagram of a fully automated HCR workflow. (A) Automated sample processing using the Opentrons Flex robot. (A1) The consumable probe plate as the starting vessel. A 96-well plate, where each well contains 5 μl of a pool of probes for four mRNA targets is used as the starting vessel for sample processing. For all liquid handling steps throughout the protocol, dispense speeds are set at 200 μl/s and aspiration speeds at 10 μl/s. All aspirations are set to use a tip to well bottom distance of 3 mm. (A2) The probe hybridization step. 10 μl of fixed embryos in hybridization buffer are transferred to each well to start the probe hybridization step. The inset illustrates transferability of sea urchin embryos using standard automation pipette tips. (A3) The probe wash step. Probes are washed from the sample using a formamide-based probe wash buffer. The insets illustrate the liquid handling speeds and tip to bottom distances allow for resuspension and mixing of embryos throughout washes. (A4) The amplification step. 10 μl of embryos are transferred to a plate of pre-plated amplifier hairpins in dextran sulfate. (A5) The amplifier wash step. Amplifier hairpins are washed using 5×SSCT, and 150 μl of samples in 5×SSCT are subsequently transferred to a glass bottom high-content imaging plate. Samples are centered using a plate shaker module set at 450 rpm for 30 min. (B) Automated confocal image acquisition using the ImageXpress HT.ai. The imaging plates containing centered samples are placed into the confocal microscope. Depending on data storage availability, the center 4 or 9 are imaged for each well. An example of an output of the demultiplexed and multiplexed versions of a single site of a positive control sample well is shown. (C) A schematic of developmental stages imaged in this study to be used as a developmental staging key. PMCs, primary mesenchyme cells.

Journal: Development (Cambridge, England)

Article Title: Automated, high-throughput in situ hybridization of sea urchin ( Lytechinus pictus ) embryos

doi: 10.1242/dev.204814

Figure Lengend Snippet: Diagram of a fully automated HCR workflow. (A) Automated sample processing using the Opentrons Flex robot. (A1) The consumable probe plate as the starting vessel. A 96-well plate, where each well contains 5 μl of a pool of probes for four mRNA targets is used as the starting vessel for sample processing. For all liquid handling steps throughout the protocol, dispense speeds are set at 200 μl/s and aspiration speeds at 10 μl/s. All aspirations are set to use a tip to well bottom distance of 3 mm. (A2) The probe hybridization step. 10 μl of fixed embryos in hybridization buffer are transferred to each well to start the probe hybridization step. The inset illustrates transferability of sea urchin embryos using standard automation pipette tips. (A3) The probe wash step. Probes are washed from the sample using a formamide-based probe wash buffer. The insets illustrate the liquid handling speeds and tip to bottom distances allow for resuspension and mixing of embryos throughout washes. (A4) The amplification step. 10 μl of embryos are transferred to a plate of pre-plated amplifier hairpins in dextran sulfate. (A5) The amplifier wash step. Amplifier hairpins are washed using 5×SSCT, and 150 μl of samples in 5×SSCT are subsequently transferred to a glass bottom high-content imaging plate. Samples are centered using a plate shaker module set at 450 rpm for 30 min. (B) Automated confocal image acquisition using the ImageXpress HT.ai. The imaging plates containing centered samples are placed into the confocal microscope. Depending on data storage availability, the center 4 or 9 are imaged for each well. An example of an output of the demultiplexed and multiplexed versions of a single site of a positive control sample well is shown. (C) A schematic of developmental stages imaged in this study to be used as a developmental staging key. PMCs, primary mesenchyme cells.

Article Snippet: All consumable plates and reagents were prepared according to the final concentrations found in the HCR RNA-FISH protocol for whole-mount sea urchin embryos (Molecular Instruments).

Techniques: Hybridization, Transferring, Amplification, Imaging, Microscopy, Positive Control